DNA synthesizers from BIOSSET are fully automated instruments for the synthesis of natural and modified oligonucleotides. They have been specially designed to meet requirements for efficiency, economy, and reliability both in the batch production of oligonucleotides and scientific research.

BIOSSET produces ASM-800
DNA synthesizer:

  • An efficient synthesis of standard, degenerate
    and modified oligonucleotides

  • Up to 16 oligonucleotides of high quality per 8 hours

  • Cost per base < $0.10

  • Easy and reliable instrument for a reasonable price



ASM-800 DNA synthesizer is a high-efficient instrument for using in a wide field of research. The instrument allows cost efficient synthesis of standard and degenerate DNA, RNA, DNA-RNA chimeras, phosphorothioate DNA and DNA containing various groups (TET, 6-FAM, HEX, biotin, Cy 3, Cy 5, etc.) to be performed. Eight different oligonucleotides can be simultaneously synthesized for 3.5 hours on eight columns. The instrument can be successfully used for the synthesis of oligonucleotides with the length up to 250 nt. The ASM-800 ensures the extremely economical consumption of reagents and solvents.


REAGENT/SOLVENT CONSUMPTION PER STANDARD SYNTHESIS
CYCLE ON EACH COLUMN

Reagent/solvent40 nmol100 nmol200 nmol400 nmol
Amidites, mg< 0.70< 0.90< 1.40< 2.20
Tetrazole (Act), mg< 0.90< 1.40< 1.90< 3.00
Capping (A + B) sol., ml0.070.100.140.20
Oxidizer, ml0.070.080.100.13
Deblocking sol., ml0.120.130.170.26
Dichlormethane, ml0.070.070.090.12
Acetonitrile, ml0.901.001.101.30



Some features of BIOSSET's DNA synthesizer:

High-precise delivery of reagents
Reagents and solvents are delivered to the synthesis columns by metering syringe pump with a flow step of 1µl and a wide range of flow rate.

Wide range of synthesis scales
Easily removable columns allow the synthesis in scales 40 - 400 nmol. The synthesis protocols are fully adaptable for each scale and ensure the accurate synthesis with minimum reagents/solvents consumption.

Thermal stability
The built-in thermostat with the heating element stabilizes reaction conditions, decreases total time of the synthesis and consumption of reagents/solvents.

Convenient set of bottles
The synthesizer is equipped with four bottles for standard phosphoramidites, four bottles for special phosphoramidites (biotin, fluorescein and others), two bottles for activators (tetrazole and the other) and eight bottles for ancillary regents/solvents. For the large scale synthesis, the bottles for activators and ancillary reagents can be easily replaced with more sizable (up to 4.3 L) bottles.

Reagent compatibility
The synthesizer successfully operates with reagents of world-wide producers: Applied Biosystems, Amersham Biosciences, Beckman, Glen Research, Prime Synthesis, Transgenomic, etc.

Friendly and flexible computer control
The synthesizer is controlled by IBM PC running under Windows 98/2000. Friendly software operates on all the synthesis stages, including run, preparation, testing, manual, and shutdown procedures. Synthesis protocols and oligonucleotide sequences are disk-stored and can be easily modified and transfered from your database to the synthesizer. Flexible programming offers an opportunity of the synthesis of new nucleic acid analogs and development of new chemistries.

Built-in diagnostics
ASM-800 is equipped with an automatic system for operative diagnostics of all units: valves, pump, thermostat, electronic control unit, etc.


SPECIFICATIONS:

SYNTHESIS CYCLE
DNA, RNA, RNA-DNA chimeras, phosphorothioate DNA, degenerate
and modified DNA
Cyanoethyl phosphoramidite
Standard synthesis scale40 / 100 / 200 / 400 nmol
SYNTHESIS MODULE
Reagent/Solvent deliveryMetering syringe pump
Step volume quantity1µl
Delivery volumeUnlimited
Delivery flow range12.5 / 25.5 / 50.0 / 100.0 µl/sec
Thermostat range25 - 40°C
ValvesBall solenoid valves
Number of monomer bottles 8 (4 for A, C, G, T and 4 extra)
Number of reagent/solvent bottles10
PERFORMANCE
Coupling efficiency> 98,5%
Cycle time (for 8 columns)7,0 - 11,0 min
Length of oligonucleotidesUp to 250 mer
GENERAL
Power requirement 110 - 240 V, 50 - 60 Hz
Dimensions (wide x deep x high)480 mm x 470 mm x 350 mm
Weight40 kg



Operating programs for PC in zip files you can find here: ASM-800 and ASM-700. The software can be run in demo mode even without synthesizer.


BIOSSET produces OPS-201
Oligonucleotides Purification System:



The OPS-201 system is an automatic device for isolation of oligonucleotides using Reverse Phase cartridges. According to HPLC data, the purity of the obtained preparations amounts to more than 90%. Purified oligonucleotides are successfully used in PCR, Genotyping, Sequencing, Hybridization assays, etc. OPS-201 is a simple and economy device in exploitation implying the use of inexpensive chemicals.




SPECIFICATIONS

Purification scale1.0 - 25.0 OD260
Lenght of oligonucleotides< 70 monomers
Purity (HPLC data)> 90 %
Cartridge quantity2
Cycle time20 - 30 min
Working gasArgon, Helium
Inlet gas pressure2.0 - 3.0 bar
Work gas pressure0.5 - 0.7 bar
Power requirements110 - 240 V, 50-60 Hz,40 VA
Overall dimensions 350 x 360 x 400 mm
Weight15 kg



OPS-201 successfully operates with RP (OPC) cartridges of world-wide producers: Applied Biosystems, Amersham Biosciences, Glen Research, Prime Synthesis, Transgenomic, etc.